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dc.contributor.authorSilva, Josué J.; et al.-
dc.contributor.otherPT_Br
dc.date.accessioned-
dc.date.accessioned2022-09-02T17:12:33Z-
dc.date.availablePT_Br
dc.date.available2022-09-02T17:12:33Z-
dc.date.copyright-
dc.date.issued2022-
dc.identifierPT_Br
dc.identifier.citationJournal of Microbiological Methods, v. 196, 106470, 2022.pt_BR
dc.identifier.urihttp://repositorio.ital.sp.gov.br/jspui/handle/123456789/430-
dc.description.abstractAflatoxins are fungal metabolites that are present as contaminants in food globally. Most aflatoxigenic species belong to Aspergillus section Flavi, and the main ones are grouped in the A. flavus clade, where many cryptic species that are difficult to discriminate are found. In this study, we investigated inter- and intraspecific diversity of the A. flavus clade to develop low-cost, species-specific PCR assays for identifying aflatoxigenic species. A total of 269 sequences of the second largest subunit of RNA polymerase II (RPB2) locus were retrieved from GenBank, and primer pairs were designed using data mining to identify A. flavus, A. parasiticus, and A. novoparasiticus. Species-specific amplicons of approximately 620, 350, and 860 bp enabled identification of target species as A. flavus, A. parasiticus, and A. novoparasiticus, respectively.pt_BR
dc.formatPT_Br
dc.languagePT_Br
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dc.rightsPT_Br
dc.sourcePT_Br
dc.subjectAspergillus novoparasiticuspt_BR
dc.subjectAspergillus section Flavipt_BR
dc.subjectAflatoxigenicpt_BR
dc.subjectSpecies-specificpt_BR
dc.subjectRPB2pt_BR
dc.titleLow-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavipt_BR
dc.typeArticlept_BR
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