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dc.contributor.authorGonçalves, Juliana Soares; et al.-
dc.contributor.otherPT_Br
dc.date.accessioned-
dc.date.accessioned2022-11-25T20:03:09Z-
dc.date.availablePT_Br
dc.date.available2022-11-25T20:03:09Z-
dc.date.copyright-
dc.date.issued2012-
dc.identifierPT_Br
dc.identifier.citationWorld J Microbiol Biotechnol, New York, v. 28, n. 4, p. 1817-1825, abril/2012.pt_BR
dc.identifier.urihttp://repositorio.ital.sp.gov.br/jspui/handle/123456789/531-
dc.description.abstractBrazil nuts are an important export market in its main producing countries, including Brazil, Bolivia, and Peru. Approximately 30,000 tons of Brazil nuts are harvested each year. However, substantial nut contamination by Aspergillus section Flavi occurs with subsequent production of aflatoxins. In our study, Aspergillus section Flavi were isolated from Brazil nuts (Bertholletia excelsa), and identified by morphological and molecular means. We obtained 241 isolates from nut samples, 41% positive for aflatoxin production. Eighty-one isolates were selected for molecular investigation. Pairwise genetic distances among isolates and phylogenetic relationships were assessed. The following Aspergillus species were identified: A. flavus, A. caelatus, A. nomius, A. tamarii, A. bombycis, and A. arachidicola. Additionally, molecular profiles indicated a high level of nucleotide variation within b-tubulin and calmodulin gene sequences associated with high genetic divergence from RAPD data. Among the 81 isolates analyzed by molecular means, three of them were phylogenetically distinct from all other isolates representing the six species of section Flavi. A putative novel species was identified based on molecular profiles.pt_BR
dc.formatPT_Br
dc.languagePT_Br
dc.language.isoenpt_BR
dc.publisherSpringerpt_BR
dc.rightsPT_Br
dc.sourcePT_Br
dc.subjectAflatoxin productionpt_BR
dc.subjectAspergillus section Flavipt_BR
dc.titleMolecular analysis of Aspergillus section Flavi isolated from Brazil nutspt_BR
dc.typeArticlept_BR
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dc.subject.cnpqPT_Br
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