Use este identificador para citar ou linkar para este item: http://repositorio.ital.sp.gov.br/jspui/handle/123456789/125
Título: Molecular analysis of Aspergillus section Nigri isolated from onion samples reveals the prevalence of A. welwitschiae
Autor(es): Massi, Fernanda Pelisson
Iamanaka, Beatriz Thie
Barbosa, Rafaella Liviero
Sartori, Daniele
Ferrranti, Larissa
Taniwaki, Marta Hiromi
Fungaro, Maria Helena Pelegrinelli







Palavras-chave: Aspergillus section Nigri
A. welwitschiae
Ochratoxin A
Fumonisin B2
Onion bulbs
Data do documento: 2020
Editor: Springer
Citação: MASSI, F. P; et al. Molecular analysis of Aspergillus section Nigri isolated from onions samples reveals the prevalence of A. welwitschiae. Brazilian Journal of Microbiology, 2020. https://doi.org/10.1007/s42770-020-00390-2.
Resumo: The aim of this study was to isolate Aspergillus section Nigri from onion samples bought in supermarkets and to analyze the fungal isolates by means of molecular data in order to differentiate A. niger and A. welwitschiae species from the other non-toxigenic species of black aspergilli, and detect genes involved in the biosynthesis of ochratoxin A and fumonisin B2. Aspergillus section Nigri were found in 98% (94/96) of the onion samples. Based on the results of multiplex PCR (performed on 500 randomly selected strains), 97.4% of the Aspergillus section Nigri strains were recognized as A. niger/A. welwitschiae. Around half of them were subjected to partial sequencing of the CaM gene to distinguish one from the other.Atotal of 97.9% of the isolates were identified as A. welwitschiae and only 2.1% as A. niger. The fum8 gene, involved in fumonisin B2 biosynthesis, was found in 36% of A. welwitschiae isolates, but radH and pks genes, involved in ochratoxin A biosynthesis, were found in only 2.8%. The presence/absence of fum8 gene in the A. welwitschiae genome is closely associated with ability/inability of the isolates to produce fumonisin in vitro. Based on these results,we suggest that in-depth studies are conducted to investigate the presence of fumonisins in onion bulbs.
URI: http://repositorio.ital.sp.gov.br/jspui/handle/123456789/125
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